Fiber Tracking

Before practicum on Friday, please complete following:

During practicum on Friday:

1.Introduction to tractography


Local fiber orientations derived from dMRI


source: source:


source: Yeh, Fang-Cheng, et al. “Tractography methods and findings in brain tumors and traumatic brain injury.” NeuroImage 245 (2021): 118651.


source: Wu, Ye, et al. “Mitigating gyral bias in cortical tractography via asymmetric fiber orientation distributions.” Medical image analysis 59 (2020): 101543.

3.Region types used in fiber tracking

4.Fiber tracking protocols


1. Combine automatic fiber tracking and region-based fiber tracking

  1. Use [AutoTract] to map left corticospinal tracts on 100206.src.gz.gqi.1.7.fib.gz

  2. Identify false results and use ROI-based fiber tracking to improve the results.

  3. [Optional] write a script to map CST in multiple FIB files(Download 3~5 FIB files)

    • rename the regions files to include “mni” in the file name, and DSI Studio will load it as MNI regions
    • examples

2. Mapping difficult pathways using region-based fiber tracking


  1. Download HCP1065 1-mm FIB file

  2. Use region-based fiber tracking, map the orbital connections in nuclei 5.

  3. Use region-based fiber tracking, map the temporal connections in nuclei 5.

  4. Use region-based fiber tracking, map the occipital connections in nuclei 5.